Functional microbes have important application potential in the remediation of contaminated sites. The research on the application of chlorobenzenes (CBs) degrader in situ remediation is still limited. This study focused on the degradation performance of CBs degrader Serratia marcescens strain TF-1 in laboratory culture and contaminated soil. TF-1 assimilated chlorobenzene (CB) with degradation rate of 0.22-0.66 mg·g _cell ^-1·h ^-1 in concentration of 20-200 mg·L ^-1. In addition, TF-1 also used sodium succinate and sodium citrate as substrates to co-metabolize CB, with degradation rates of 0.26-2.00 and 0.31-1.72 mol·g _cell ^-1·h ^-1, respectively. The addition of TF-1 and sodium succinate in contaminated soil can remarkably promote the removal of CBs. Finally, combination of biostimulation and bioaugmentation using sodium succinate and TF-1 were carried out in a test field and CBs were almost completely removed by TF-1 within 10 d. Sequence analysis suggested that TF-1 colonized successfully in field soil, and the indigenous microorganism Azoarcus could be enriched. The novel strain was used for in-situ CBs biodegradation for the first time, and the results provide guidance for remediation of organic contaminated soil.