Expression data of FOS and JUN genes and FTIR spectra provide diagnosis
of thyroid carcinoma
Abstract
We explore the feasibility of using FOS and JUN gene expression and
ATR-FTIR † for diagnosis of thyroid cancer. For the
study, 38 samples (6 non-neoplastic (NN), 10 papillary thyroid carcinoma
(PTC), 7 follicular thyroid carcinoma (FTC), and 15 benign tumors (BT)
were subjected to RNA extraction followed by quantitative real time PCR
(qRT-PCR) and 30 samples (5 NN, 9 PTC, 5 FTC, and 11 benign) were used
for Attenuated Total Reflectance – Fourier Transform Infrared
(ATR-FTIR) followed by multivariate analysis. We found FOS and JUN
expression in malignant tumor samples to be significantly lower than NN
and benign. ATR-FTIR after multivariate analysis could identify the
difficult to diagnose PTC § with 93% efficiency.
Overall, results suggest the diagnostic potential of molecular biology
techniques combined with ATR-FTIR spectroscopy in differentiated thyroid
carcinomas (PTC and FTC) and benign tumors.