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Using eDNA to survey amphibians: Methods, applications, and challenges
  • +1
  • Xiaoxuan Sun,
  • Ningning Guo,
  • Jianan Gao,
  • Nengwen Xiao
Xiaoxuan Sun
Chinese Research Academy of Environmental Sciences
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Ningning Guo
Chinese Research Academy of Environmental Sciences
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Jianan Gao
Chinese Research Academy of Environmental Sciences
Author Profile
Nengwen Xiao
Chinese Research Academy of Environmental Sciences

Corresponding Author:[email protected]

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Abstract

In recent years, environmental DNA (eDNA) has received attention from biologists due to its sensitivity, convenience, labor and material efficiency, and lack of damage to organisms. The extensive application of eDNA has opened avenues for the monitoring and biodiversity assessment of amphibians, which are frequently small and difficult to observe in the field, in areas such as biodiversity survey assessment and detection of specific, rare and endangered, or alien invasive species. However, the accuracy of eDNA can be influenced by factors such as ambient temperature, pH, and false positives or false negatives, which makes eDNA an adjunctive tool rather than a replacement for traditional surveys. This review provides a concise overview of the eDNA method and its workflow, summarizes the differences between applying eDNA for detecting amphibians and other organisms, reviews the research progress in eDNA technology for amphibian monitoring, identifies factors influencing detection efficiency, and discusses the challenges and prospects of eDNA. It aims to serve as a reference for future research on the application of eDNA in amphibian detection.
18 Aug 2023Submitted to Biotechnology and Bioengineering
19 Aug 2023Submission Checks Completed
19 Aug 2023Assigned to Editor
19 Aug 2023Review(s) Completed, Editorial Evaluation Pending
19 Aug 2023Reviewer(s) Assigned
07 Oct 2023Editorial Decision: Revise Minor
24 Oct 20231st Revision Received
24 Oct 2023Submission Checks Completed
24 Oct 2023Assigned to Editor
24 Oct 2023Review(s) Completed, Editorial Evaluation Pending
25 Oct 2023Editorial Decision: Accept