GC-MS analysis:
Fatty acids profile of all the samples used in the study was determined
before and after thermal heating. The GC-MS analyses were done according
to procedure previously described in details by Resende et. al 2020. Gas
chromatography-mass spectrometry analyses were performed using an GC-MS
system SCION™ (Bruker, USA), GC-436. Detection was carried out with SQ
mass-selective single quadrupole detector. The GC-MS operation control
and data process were carried out by Bruker MS Workstation 8 SP2 for
SCION™ software package. The injector temperature was 280 °C. The oven
temperature was held at 70 °C for 1 min, then increased to 270 °C at a
heating rate 7 °C / min. The carrier gas was helium (purity 99.999%) at
flow rate 1.6 ml/ min. The sample volume was 1 µl. The conditions for
election impact ionization (EI) were an ion energy of 70 eV and mass
range scanning was 39-500 m/z.