Preparation of linseed emulsion:
Twenty five grams of linseed seeds (Linum usitatissimum ) were
grounded and homogenated together with distillated water (300 ml) three
times for 10 minutes. The mixture was filtered through gauze and then it
was put in the centrifuge (x7000 rpm) for 20 min. The top liquid
supernatant phase of the mixture was transferred to a new bottle. The
bottle was then sonicated on ice for 6 minutes, and after 5 minutes of
break it was sonicated again for more 6 minutes.