Thermal oxidation Procedure
Thermal oxidation experimental design was based on previous studies
(Resende et al., 2020). The samples were autoxidized by heating 100 ml
samples in a 250 ml beaker on a hot plate at 55 °C. Air was pumped into
the beaker with maximum magnetic stirring for 96 hours. Before the
induced autoxidation process (time 0) and after 24, 48, 72 and 96 hours
a 20 ml sample was removed into a vial which was placed in cold water
for 10 min and then stored sealed at −20 °C to avoid further oxidation
prior to analysis.