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\begin{document}

\title{Cell-derived and enzyme-based decellularized extracellular matrix
exhibit compositional and structural differences that are relevant for
its use as a biomaterial}



\author[1]{Svenja Nellinger}%
\author[2]{Ivana Mrsic}%
\author[3]{Silke Keller}%
\author[1]{Simon Heine}%
\author[3]{Alexander Southan}%
\author[4]{Monika Bach}%
\author[1]{Ann-Cathrin Volz}%
\author[2]{Thomas Chassé}%
\author[1]{Petra Kluger}%
\affil[1]{Reutlingen University}%
\affil[2]{University of Tübingen}%
\affil[3]{University of Stuttgart}%
\affil[4]{University of Hohenheim}%


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  \date{June 09, 2024}


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\begin{abstract}
Due to its availability and minimal invasive harvesting human adipose
tissue-derived extracellular matrix (dECM) is often used as a
biomaterial in various tissue engineering and healthcare applications.
Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated
from in vitro cultured cells. So far both types of ECM were investigated
extensively towards their application as (bio)material in tissue
engineering and healthcare. However, a systematic characterization and
comparison of soft tissue dECM and cdECM is still missing. In this
study, we characterized dECM from human adipose tissue, as well as cdECM
from human adipose-derived stem cells (ASCs), towards their molecular
composition, structural characteristics, and biological purity. The dECM
was found to exhibit higher levels of collagens and lower levels of
sulfated glycosaminoglycans (sGAGs) compared to cdECMs. Structural
characteristics revealed an immature state of the fibrous part of cdECM
samples. By the identified differences, we aim to support researchers in
the selection of a suitable ECM-based biomaterial for their specific
application and the interpretation of obtained results.%
\end{abstract}%



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