3.2 pCIgD-Man-L induces viral specific proliferation of lymphocytes in mice
A lymphoproliferative assay was performed to study the cellular immune response against the viral antigen. After splenocytes extraction from mice, they were stimulated in vitro with iBoHV-1. As a result, the percentages of specific lymphoproliferation was higher in pCIgD-Man-L group (p<0.05) than in pCIgD group(Fig.2) . Taking together, these results indicate that pCIgD vaccine formulated with liposome Man-L could induce a higher humoral and cellular response against BoHV-1 in mice. Therefore, this formulation was further tested in a trial in bovines, natural host species.