3.2 pCIgD-Man-L induces viral specific proliferation of
lymphocytes in mice
A lymphoproliferative assay was performed to study the cellular immune
response against the viral antigen. After splenocytes extraction from
mice, they were stimulated in vitro with iBoHV-1. As a result,
the percentages of specific lymphoproliferation was higher in
pCIgD-Man-L group (p<0.05) than in pCIgD group(Fig.2) . Taking together, these results indicate that pCIgD
vaccine formulated with liposome Man-L could induce a higher humoral and
cellular response against BoHV-1 in mice. Therefore, this formulation
was further tested in a trial in bovines, natural host species.