3.1 pCIgD-Man-L increased specific BoHV-1 antibodies in mouse
In order to study the humoral immune response induced by pCIgD-Man-L vaccine, an ELISA test to detect anti-BoHV-1 antibodies was used. Vaccines pCIgD and pCIgD-Man-L were capable to induce specific sera antibody at 20 and 40 days after administration, but the formulation with liposome improved notably the IgG titer with respect to pCIgD (p<0.01, p<0.05 respectively). In addition, it was possible to detect titers at 120 days; indeed, in case of pCIgD-Man-L, antibody titer levels remained higher (p<0.001) with respect to those observed on the pCIgD treated group, where the titer declined (Fig.1a ).
When the profile of antibody isotype was analyzed, a marked difference between the group treated with pCIgD-Man-L and the group treated with pCIgD alone was observed. Group treated with pCIgD-Man-L had elevated IgG1 (p<0.001), IgG2a (p<0.05) and IgG2b (p<0.01) levels with respect to the group treated with pCIgD (Fig.1b ).