Partial G gene sequencing
The partial G gene amplicons obtained were purified using ExoSAP-IT™Express PCR Product Cleanup (Thermo Fisher Scientific, Massachusetts, USA) according to the manufacturer’s instructions and sequenced in both directions using RT-nested PCR primers G5- (5′- CAAAGAGCCAATAAGCCCA-3′) and G9+B (5′-TAGTCCTCAAGCAAGTCCTC-3′), by a commercial sequencing service (Macrogen Europe, Amsterdam, The Netherlands). The obtained sequences were named using the following nomenclature: aMPV/B/Country of origin/Host species (Turkey: Ty, Chicken: Ck or Guinea fowl)/sample ID number/year of detection.