Partial G gene sequencing
The partial G gene amplicons obtained were purified using ExoSAP-IT™Express PCR Product Cleanup (Thermo Fisher Scientific,
Massachusetts, USA) according to the manufacturer’s instructions and
sequenced in both directions using RT-nested PCR primers G5- (5′-
CAAAGAGCCAATAAGCCCA-3′) and G9+B (5′-TAGTCCTCAAGCAAGTCCTC-3′), by a
commercial sequencing service (Macrogen Europe, Amsterdam, The
Netherlands). The obtained sequences were named using the following
nomenclature: aMPV/B/Country of origin/Host species (Turkey: Ty,
Chicken: Ck or Guinea fowl)/sample ID number/year of detection.