Material and Methods
During the nationwide comprehensive monitoring of wild boars from
October 2 to December 30, 2019, 56 viral isolates were collected from
whole blood or tissue of wild boar carcasses in the border area covering
Paju and Yeoncheon counties of Gyeonggi Province and Cheorwon county of
Gangwon Province by NIER (Fig. 1). The positive rate was highest in Paju
(Table 1). Partial segments of B646L (p72) and EP402R(CD2v) as well as an intergenic region (IGR) between I73R andI329L were amplified from each of the viral DNAs by PCR using
specific primer sets, as described previously (Kim S. H. et al., 2020).
The PCR products were subject to automated paired-end sequencing
reactions and the resulting contig sequences were used in phylogenetic
analyses together with their homologues retrieved from the GenBank
database (neighbour-joining algorithm with the MEGA 6.0 software).