Figures legends
Figure 1. A Tequila Bat (Leptonycteris yerbabuenae) visiting a Cactus flower (Image for journal cover)
Figure 2. Clamps used for this study. The cacti-specific chloroplast clamp (cpPNA) has a single base modification at the 11th position (T C, Fitzpatrick et al (2018)) compared with the universal chloroplast clamp (pPNA). Both universal clamps are based on the study of Lundberg et al (2013).
Figure 3. PCR protocol for the implementations of the cpPNA-DNA clamps. I- Normal workflow according to the Earth Microbiome Protocol (Caporaso et al. 2012); II. Our modified protocol with the addition of one extra step for clamp annealing (Step b).
Figure 4. Percentage of reads kept after the filtering out of the chloroplast and mitochondrial DNA using QIIME2. Bat samples were clamped by implementing cpPNA/mPNA and birds by using pPNA/mPNA. The Zymo Mock community was treated with cpPNA/mPNA.