DNA extraction
We extracted the faecal pellets by using the NucleoSpin® Soil extraction
kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s
guidelines. For the Tequila bat samples, we homogenized the sample (2 x
150 sec at 50 Hz) by using a SpeedMill PLUS (Analytik Jena, Jena,
Germany). To maximize DNA yield, we conducted consecutive elutions (2 x
50 µl) with a preheated (ca. 45°C) SE buffer. For the mockingbird
samples, the samples were washed in 50 µl SE Buffer and then homogenized
using the same procedure as with the Tequila bat samples. We stored the
extracted DNA at -20°C.