Figures legends
Figure 1. A Tequila Bat (Leptonycteris yerbabuenae) visiting a Cactus
flower (Image for journal cover)
Figure 2. Clamps used for this study. The cacti-specific chloroplast
clamp (cpPNA) has a single base modification at the
11th position (T C, Fitzpatrick et al (2018)) compared
with the universal chloroplast clamp (pPNA). Both universal clamps are
based on the study of Lundberg et al (2013).
Figure 3. PCR protocol for the implementations of the cpPNA-DNA clamps.
I- Normal workflow according to the Earth Microbiome Protocol (Caporaso
et al. 2012); II. Our modified protocol with the addition of one extra
step for clamp annealing (Step b).
Figure 4. Percentage of reads kept after the filtering out of the
chloroplast and mitochondrial DNA using QIIME2. Bat samples were clamped
by implementing cpPNA/mPNA and birds by using pPNA/mPNA. The Zymo Mock
community was treated with cpPNA/mPNA.