FIGURE 2
Figure 2: Proportion of seroreactive animals according to the
locations that samples were collected.
From the 20 samples destined to culturing leptospires, only one
retrieved viable leptospires in selective medium. The strain was named
FN115 and further molecular and serological characterization was
performed. Serogrouping of the FN115 strain revealed a strong and
specific titration against serogroup Pyrogenes (12,800 for serogroup
Pyrogenes, 1,600 for Icterohaemorrhagiae, and 800 for serogroup
Canicola). The MLST analysis revealed that the strain FN115 belongs to
sequence type 37 of the MLST scheme, which comprises only L.
interrogans strains belonging to serogroup Canicola and Pyrogenes, all
recovered from dogs and human hosts. All loci presented 100% identity
in the BLAST search except sucA, which presented only one
mismatch in a homopolymeric region, which are particularly prone to
error in Ion Torrent PGM platform.