2.1. Reagents
All chemicals were purchased from Sigma-Aldrich (Madrid, Spain), unless
otherwise stated.
2.2 Preparation and administration of L. fermentum CECT5716
The probiotic Lactobacillus fermentum CECT5716 was provided by
Biosearch, S.A. (Granada, Spain) and grown in De Man, Rogosa and Sharpe
(MRS) media for a period of 24 h at 37 °C under anaerobic conditions
using the Anaerogen system (Oxoid, Basingstoke, UK). For probiotic
treatment, it was suspended using a sterile PBS solution and was diluted
to obtain a concentration of 5 × 109 CFUs in 1 ml.