2.4.1. Glucose tolerance test
One week before the sacrifice, a glucose tolerance test was performed on mice fasted for 8 h. They received a 50% glucose solution in water at a dose of 2 g/kg of body weight by intraperitoneal injection, and blood was collected from the tail vein at 0, 15, 30, 60 and 120 min after injection. Blood glucose was measured using a handheld glucometer (Contour XT, Ascensia Diabetes Care, S.L., Barcelona, Spain).