2.10. Statistics
All results are expressed as the mean ± SEM. Differences between means
were assessed for statistical significance with a one-way analysis of
variance (ANOVA) and post-hoc least significance tests. Differences
between proportions were examined with the chi-squared test. All
statistical analyses were performed with the GraphPad 8 software package
(GraphPad Software, Inc., La Jolla, CA, USA), with statistical
significance set at p< 0.05 .