Figure
legends
Figure 1 T-cell subset variations in various tissues upon
moderately virulent ASFV infection. At the indicated time points,
domestic pigs (orange) and wild boar (blue) were euthanized and
lymphocytes of the respective tissues were isolated.
CD3+ lymphocytes were subdivided into γδ T cell
receptor (TCR)– αβ T cells and γδ
TCR+ γδ T cells. Frequency of (A ) αβ and
(B ) γδ T cells in blood, spleen, lung, liver, and ghLN. Each
point represents data from a single pig while bars represent the means
for the designated time points. Data from trial 1 (●), data from trial 2
(■). *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 2 Increasing frequencies of CD8α+ αβ T
cells. At the indicated time points, domestic pigs (orange) and wild
boar (blue) were euthanized and lymphocytes of the respective tissues
were isolated. CD3+ αβ T cells were analyzed for CD4
and CD8α expression. Frequency of (A )
CD4–/CD8α+ αβ T cells and
(B ) CD4+/CD8α+ αβ T cells
in blood, spleen, lung, liver, and ghLN. Each point represents data from
a single pig while bars represent the means for the designated time
points. Data from trial 1 (●), data from trial 2 (■). *, p <
0.05. ghLN, gastro-hepatic lymph node
Figure 3 Increasing
frequencies of CD8α+ effector γδ T cells in wild
boar. At the indicated time points, domestic pigs (orange) and wild
boar (blue) were euthanized and lymphocytes of the respective tissues
were isolated. CD3+ γδ T cells were analyzed for CD8α
expression. Frequency of CD8α+ γδ T cells (A )
spleen, (B ) lung, (C ) liver, and (D ) ghLN.
Each point represents data from a single pig while bars represent the
means for the designated time points. Data from trial 1 (●), data from
trial 2 (■). *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 4 Increasing frequencies of proliferating
CD8αα+ and CD8αβ+ αβ T cells. At
the indicated time points, domestic pigs (orange) and wild boar (blue)
were euthanized and lymphocytes of the respective tissues were isolated.
CD8α+ αβ T cells were subdivided based on their
expression of the CD8β chain. Frequency of (A )
CD8αα+ and (B ) CD8αβ+ T
cells in blood, spleen, lung, and liver. Each point represents data from
a single pig while bars represent the means for the designated time
points. *, p < 0.05.
Figure 5 Loss of perforin
expression in cytotoxic T cells. At the indicated time points, domestic
pigs (orange) and wild boar (blue) were euthanized and lymphocytes were
isolated. (A ) CD8αα+ and
CD8αβ+ αβ T cells, and (B )
CD2+/CD8α+ effector γδ T cells were
investigated for perforin expression in the indicated tissues. Each
point represents data from a single pig while bars represent the means
for the designated time points. Data from trial 1 (●), data from trial 2
(■). *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 6 T-bet-dependent
T-cell activation in wild boar. At the indicated time points, wild boar
were euthanized and lymphocytes were isolated. (A )
CD4+/CD8α–,
CD4–/CD8α+, and
CD4+/CD8α+ αβ T cells (from left to
right) as well as (B ) activated and effector γδ T cells were
analyzed for their expression of T-bet. T-bet expression in domestic
pigs for comparison can be found in Supplemental figure 2. Each point
represents data from a single pig while bars represent the means for the
designated time points. *, p < 0.05. ghLN, gastro-hepatic
lymph node
Figure 7 FoxP3+ regulatory T cells appeared in
domestic pigs and wild boar. At the indicated time points, domestic
pigs (orange) and wild boar (blue) were euthanized and lymphocytes of
the respective tissues were isolated.
CD4+/CD8α– or
CD4+/CD8α+ αβ T cells were analyzed
for their expression of FoxP3. Frequency of (A )
FoxP3+/CD4+/CD8α–and (B )
FoxP3+/CD4+/CD8α+T cells in blood, spleen, and ghLN. Each point represents data from a
single pig while bars represent the means for the designated time
points. *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 8 Activation and differentiation of iNKT cells in
domestic pigs. At the indicated time points, domestic pigs were
euthanized and lymphocytes of the respective tissues were isolated.
CD3+ mCD1d tetramer+ iNKT cells were
analyzed by flow cytometry. Frequency of iNKT cells positive for
(A ) ICOS, (B ) MHC II, (C ) CD8α, and
(D ) CD4/CD8α. Each point represents data from a single pig
while bars represent the means for the designated time points. *, p
< 0.05. ghLN,
gastro-hepatic lymph node
Supplemental figure 1 Proliferation of
CD4+/CD8α— and
CD4+/CD8α+ αβ T cells in the
spleen. At the indicated time points, domestic pigs (orange) and wild
boar (blue) were euthanized and lymphocytes of the respective tissues
were isolated. Frequency of (A )
CD4+/CD8α— and (B )
CD4+/CD8α+ T cells in spleen. Each
point represents data from a single pig while bars represent the means
for the designated time points. *, p < 0.05.
Supplemental figure 2 No evidence of T-bet-dependent activation
in domestic pigs. At the indicated time points, domestic pigs (orange)
were euthanized and lymphocytes of the respective tissues were isolated.
Frequency of T-bet+ cells in (A ) blood,
(B ) spleen, (C ) liver, and (D ) ghLN. Each
point represents data from a single pig while bars represent the means
for the designated time points. *, p < 0.05. ghLN,
gastro-hepatic lymph node
Supplemental figure 3 Frequency and CD25 expression of iNKT
cells in domestic pigs. At the indicated time points, domestic pigs
were euthanized and lymphocytes of the respective tissues were isolated.
CD3+ mCD1d tetramer+ iNKT cells were
analyzed by flow cytometry. Frequency of iNKT cells (A ) of
CD3+ cells and (B ) positive for CD25. Each
point represents data from a single pig while bars represent the means
for the designated time points. *, p < 0.05. ghLN,
gastro-hepatic lymph node