Walking
When walking is considered as an ordinal score (Immobile < With difficulty < Normal), there was a highly significant Treatment × Day interaction (P = 1.8×10–13). Initially (Day 0), there were no differences in walking scores between the three groups (all P > 0.15) and . some cattle remained ‘Immobile’ or ‘With difficulty’ throughout the study period (Figure 5). However, walking improved for the two treatment groups, particularly for the TS treatment group. However, the differences between these two groups never reached threshold significance (allP > 0.05). There were no significant effects of other factors on walking (Age: P = 0.583; Breed: P = 0.9012; Sex: P = 0.778).