2.4. Aggregate spike preparations
Aggregate solutions for aggregate spike experiments were prepared as
follows. mAb aggregate solution was produced by incubating 10 mg/mL mAb
in 0.1 M NaCl, pH 4 at 60 ℃ for 60 min, which produced a spike stock
solution with aggregate content of 11.4% dimer and 33.3% trimer or
larger species.
Plasma IgG aggregate solution was prepared by mixing 5.0 mg/mL IgG in
0.1 M glycine, adjusting to pH 2 and incubating at room temperature for
1 h. The produced aggregate spike solution had aggregate content of
14.8% dimer and 57.1% trimer or larger species.