Experimental Approach
TKP metabolites level were determined using HPLC and LC-MS/MS in the
hippocampal samples of patients with MTLE-HS compared to autopsy and
non-seizure control samples. mRNA and protein expression of TKP enzymes
were determined by qPCR and western blot. Spontaneous glutamatergic
activities were recorded from pyramidal neurons in presence of
kynurenine (KYN) and KYNA using whole cell patch clamp.