Histopathological examinations revealed evidence of hippocampal
sclerosis
Haematoxylin-Eosin staining of the hippocampal sections obtained from
patients with MTLE-HS revealed typical sclerotic hippocampus consists of
massive cell loss, cystic cavities, neuronal degeneration, gliosis and
infiltration of inflammatory cells (Figure 1a). The NeuN
immunohistochemistry confirms pyramidal neuron loss, presence of
ischemic neurons and abnormal neuronal architecture (Figure 1b). Another
hallmark of the hippocampal sclerosis is reactive astrogliosis with
hypertrophic astrocytes manifesting distinctive GFAP staining and thick,
long projections (Figure 1c). In autopsy control tissue sections,
negligible neuronal loss was observed (Figure 1d and e). The GFAP
immunohistochemistry did not reveal any reactive astrogliosis (Figure
1f). These data suggested that architectural alterations like, pyramidal
cell apoptosis, necrosis and reactive astrogliosis were observed in the
hippocampal samples of the MTLE-HS patients.