5. Conclusion
In this study,
the
mechanism of SLs sedimentation in multiphase fermentation process was
elucidated for the ratio of O/S. And then
UEST
was introduced to strengthen the SLs sedimentation rate.
The
real-time rational in-situ separation of SLs and semi-continuous
fermentation with UEST was accomplished to achieve productivity and
yield of 2.15 g/L and 0.58 g/g respectively as well as reduce the losses
of cells, glucose, and rapeseed oil by 68.2%, 16.2%, and 65.5%,
respectively. The rational in-situ separation and UEST for
complex multiphase systems could be extended to other biosurfactant
fermentation systems to improve production efficiency.