Microwave Method |
Weigh the plant powder and mix with the appropriate
amount of extractant. After soaking for 30 minutes, immerse the glass
tube and the sample in the water of the microwave device, set the
parameters and then start the extraction. |
5.8 mg/g ursolic acid
extracted from the fruit of Ligustrum lucidum
|
33 |
Rapid Solvent Extraction |
The fast solvent method can be implemented
using systems introduced by related companies, such as Dionex ASE 100.
The samples were placed in the extraction tank and extracted in two
static cycles at 40 ℃ and 60 ℃ under 10MPa pressure. |
9.4 mg/g dry
weight ursolic acid extracted from leaves of Thymus mongolicus
Ronn. |
35 |
Impregnation |
Wash the fruit and leaves with distilled water, then air
dry for a week to remove all water. At 258 ℃, soak in anhydrous alcohol
for 48 hours, shake it irregularly and repeat twice. Among them, 20 ml/g
ethanol was used in leaves, 2 ml/g ethanol was used in fruits, and then
ethanol extract was obtained by filtration. |
0.46-1.08 and 1.99-4.54
mg/g dry weight ursolic acid extracted from the fruits and leaves of
Morocco nuts respectively |
39 |
Soxhlet Extraction |
Degrease 4 parts of 2.00g aliquots in Soxhlet
extraction unit for 4 hours. After drying, 80% hot methanol extract
material was used, and ethanol extract and methanol extract were
collected and concentrated in a rotating evaporator at 60 ℃. Add
deionized water and stir thoroughly, and introduce the extract into the
extraction column. Then the column is washed with butanol soaked with
water, and the filtrate is evaporated to dryness in 70 ℃ evaporator |
20.53 mg/g ursolic acid extracted from flowers of Calendula
officinalis L.
|
40 |
Hot Reflux Method |
The dried plant material was mixed with 100 mL of
acetone. Connect with cooling water and heat in a 60°C water bath for 30
minutes, and repeat three times with new solvent. Finally, the extract
was collected, filtered and concentrated. |
92.6 mg/g ursolic acid
extracted from Lamium album
|
41 |
Ultrasonic Method |
First, the ground material and solvent are mixed
into the flask, and the flask is placed in a device that can provide
ultrasonic wave. The parameters are adjusted, and the mixing of solvent
and solid material is promoted by the diffusion of ultrasonic vibration
energy. |
9.11 mg/g ursolic acid extracted from flowers of Punica
granatum L.
|
42 |
Supercritical Fluid Extraction |
First, the liquid carbon dioxide is
pressurized to the required pressure in the coolant pump while being
heated and reaches the working temperature before reaching the
extractor. After flowing through the extractor pre-loaded with sample,
the cooled ethanol is used as a collector for the extraction liquid, and
the effluent of the extractor is depressurized by a heated back pressure
regulator. The solvent was then evaporated in a nitrogen stream on a
rotary evaporator, evaporated in a vacuum oven for 6 hours, and the
temperature was 60°C. |
1.8 mg/g ursolic acid extracted from
Eucalyptus grandis
|
43 |