Method Extraction and purification Result Reference
Microwave Method Weigh the plant powder and mix with the appropriate amount of extractant. After soaking for 30 minutes, immerse the glass tube and the sample in the water of the microwave device, set the parameters and then start the extraction. 5.8 mg/g ursolic acid extracted from the fruit of Ligustrum lucidum 33
Rapid Solvent Extraction The fast solvent method can be implemented using systems introduced by related companies, such as Dionex ASE 100. The samples were placed in the extraction tank and extracted in two static cycles at 40 ℃ and 60 ℃ under 10MPa pressure. 9.4 mg/g dry weight ursolic acid extracted from leaves of Thymus mongolicus Ronn. 35
Impregnation Wash the fruit and leaves with distilled water, then air dry for a week to remove all water. At 258 ℃, soak in anhydrous alcohol for 48 hours, shake it irregularly and repeat twice. Among them, 20 ml/g ethanol was used in leaves, 2 ml/g ethanol was used in fruits, and then ethanol extract was obtained by filtration. 0.46-1.08 and 1.99-4.54 mg/g dry weight ursolic acid extracted from the fruits and leaves of Morocco nuts respectively 39
Soxhlet Extraction Degrease 4 parts of 2.00g aliquots in Soxhlet extraction unit for 4 hours. After drying, 80% hot methanol extract material was used, and ethanol extract and methanol extract were collected and concentrated in a rotating evaporator at 60 ℃. Add deionized water and stir thoroughly, and introduce the extract into the extraction column. Then the column is washed with butanol soaked with water, and the filtrate is evaporated to dryness in 70 ℃ evaporator 20.53 mg/g ursolic acid extracted from flowers of Calendula officinalis L. 40
Hot Reflux Method The dried plant material was mixed with 100 mL of acetone. Connect with cooling water and heat in a 60°C water bath for 30 minutes, and repeat three times with new solvent. Finally, the extract was collected, filtered and concentrated. 92.6 mg/g ursolic acid extracted from Lamium album 41
Ultrasonic Method First, the ground material and solvent are mixed into the flask, and the flask is placed in a device that can provide ultrasonic wave. The parameters are adjusted, and the mixing of solvent and solid material is promoted by the diffusion of ultrasonic vibration energy. 9.11 mg/g ursolic acid extracted from flowers of Punica granatum L. 42
Supercritical Fluid Extraction First, the liquid carbon dioxide is pressurized to the required pressure in the coolant pump while being heated and reaches the working temperature before reaching the extractor. After flowing through the extractor pre-loaded with sample, the cooled ethanol is used as a collector for the extraction liquid, and the effluent of the extractor is depressurized by a heated back pressure regulator. The solvent was then evaporated in a nitrogen stream on a rotary evaporator, evaporated in a vacuum oven for 6 hours, and the temperature was 60°C. 1.8 mg/g ursolic acid extracted from Eucalyptus grandis 43