3.1 Establishing the fluorescent LAMP assay
The fluorescent LAMP assay was used to screen the optimal primers from
the designed 5 sets of primers, the concentration of primers and the
reaction temperature. According to the application plot, the second set
of primer has the highest efficiency and was selected to perform the
LAMP assay (Supplementary Figure S1), the primers were shown in Table 1.
The optimal concentration of outer primers (F3/B3) and inner primers
were 100 nM and 600 nM (Supplementary Figure S2), respectively. The
optimal LAMP reaction temperature was 63 oC
(Supplementary Figure S3). In addition, the results also showed that the
fluorescent LAMP assay can detect 7 copies/μl with the aid of ABI
QuantStudio 5.