2.3 Oligonucleotide primers for LAMP
Oligonucleotide primers specific for ASFV were designed based on its p72 gene. Multiple sequences of p72 were aligned using MegAlign of the DNASTAR program (Version 7.1, DNASTAR, Madison, WI, USA). The most conserved region of the gene was subjected to design LAMP primers using the PrimerExplorer V4 software (http://primerexplorer.jp). The LAMP primers comprised two outer primers F3, B3 and two inner primers FIP, BIP. Five sets of primers were designed.