Culture medium |
Combining the FBA with Plackett Burman (PB) for a
recombinant Escherichia coli
|
Identifying the key amino acids
and carbon sources for successful improvement in siderophore production |
[67] |
Culture medium |
Using the FVSEOF algorithm to design feeding strategies
for CHO cells |
Suggesting threonine and arachidonate as supplements by
the metabolic model and a two-fold increase in antibody production |
[68] |
Culture medium |
Designing a strategy for amino acid supplementation by
a stoichiometric model |
Enhancing the production of interleukin-2 by
adding a mixture of leucine, aspartic acid, and glycine |
[69] |
Culture medium
|
Performing FBA for C. sporogenes NCIM on glucose, glycerol &
their mixture
|
Glucose and glucose-glycerol mixture improve the growth and the butyric
acid production.
Glycerol reduces the growth and improves ethanol yield
|
[71]
|
pH |
MFA used for Bacillus licheniformis to study the effect of
pH on metabolic flux distribution |
Proposing a pH-controlled strategy
to improve β-lactamase production |
[8] |
pH |
FBA used to gain mechanistic insight into the question of how pH
affects lactate metabolism for a mammalian cell |
Lowering the pH
increases lactate consumption, antibody production and makes the cell
more energy-efficient |
[72] |
Temperature |
FBA implemented to study the effect of mild hypothermia
conditions on the CHO cell behaviors |
Formation of premature
glycosylated antibodies during the stationary phase at 32 °C |
[73] |