Figure 3. The amplification efficiency of different LNA-mediated real-time PCR systems and the blank control (without LNA) were evaluated. Serially diluted standard templates at different mutation proportions of 1%, 0.1% and 0% (in 10000 copies of wild-type gene) were tested. Real-time PCR plots of different LNA systems at 0% (A), 1% (B), and 0.1% (C) mutant DNA. (D) Line chart of the mean Ct (threshold cycles) value calculated from three individual replicates. (E) The melting curves of different LNA clamps with mutant sequences and (F) wild-type sequence.