To allow the free movement of Volvox colonies, a sample area (see image below) was constructed. A square chip of outer dimensions 25 mm x 25 mm with a 7.75 mm x 7.75 mm square cut from the middle was 3D printed in PLA and attached to a standard glass microscope slide using superglue adhesive. This square well has depth of 1.5 mm, allowing Volvox colonies of 350–500 µm in diameter to move freely in the x-y plane, with some limited movement in the z plane.