Figure Legends
Figure 1. Time course profile of P. tricornutum biomass concentration in Photo1 (P1): Photoautotrophic control culture with additions of 882 µM sodium nitrate (NaNO3);Photo 2 (P2): Photoautotrophic control culture with additions of 441 µM urea and 882 µM sodium nitrate (NaNO3);Photo 3 (P3): Photoautotrophic control culture with additions 441 µM of urea; Mixo 1 (M1): Mixotrophic culture with additions of 0.1M glycerol and 882 µM sodium nitrate (NaNO3);Mixo 2 (M2): Mixotrophic culture with additions of 0.1M glycerol, 441 µM of urea and 882 µM sodium nitrate (NaNO3); Mixo 3 (M3): Mixotrophic culture with additions of 0.1M glycerol and 441 µM of urea; Mixo 4 (M4):Mixotrophic culture with additions of 0.1M glycerol, feeded with 441 µM of urea post 36 hours of cultivation. Values indicate mean (n = 3) with standard error; * indicate statistical significance by one-way analysis of variance (ANOVA), p-value < 0.05.
Figure 2A. Quantification of EPA content (mg g-1) of P. tricornutum cultivated in different conditions, Photo1 (P1): Photoautotrophic control culture with additions of 882 µM sodium nitrate (NaNO3);Photo 2 (P2): Photoautotrophic control culture with additions of 441 µM urea and 882 µM sodium nitrate (NaNO3);Photo 3 (P3): Photoautotrophic control culture with additions 441 µM of urea; Mixo 1 (M1): Mixotrophic culture with additions of 0.1M glycerol and 882 µM sodium nitrate (NaNO3);Mixo 2 (M2): Mixotrophic culture with additions of 0.1M glycerol, 441 µM of urea and 882 µM sodium nitrate (NaNO3); Mixo 3 (M3): Mixotrophic culture with additions of 0.1M glycerol and 441 µM of urea. Values indicate mean (n = 3) with standard error; * indicate statistical significance by one-way analysis of variance (ANOVA), pvalue < 0.05.
Figure 2B. Quantification of fucoxanthin content (mg g-1) of P. tricornutum cultivated inPhoto1 (P1): Photoautotrophic control culture with additions of 882 µM sodium nitrate (NaNO3); Photo 2 (P2):Photoautotrophic control culture with additions of 441 µM urea and 882 µM sodium nitrate (NaNO3); Photo 3 (P3):Photoautotrophic control culture with additions 441 µM of urea;Mixo 1 (M1): Mixotrophic culture with additions of 0.1M glycerol and 882 µM sodium nitrate (NaNO3); Mixo 2 (M2): Mixotrophic culture with additions of 0.1M glycerol, 441 µM of urea and 882 µM sodium nitrate (NaNO3); Mixo 3 (M3): Mixotrophic culture with additions of 0.1M glycerol and 441 µM of urea conditions. Values indicate mean (n = 3) with standard error; * indicate statistical significance by one-way analysis of variance (ANOVA), pvalue < 0.05.