2.1.1. Epimastigote forms
Epimastigote forms were cultured at 28 ÂșC in Gibco RPMI 1640 medium supplemented with 10% (v /v ) heat-inactivated fetal bovine serum (hiFBS), 0.03 M hemin and 0.5% (w /v ) BBL trypticase (Kendall et al. , 1990).