2.4.3. Immunosuppression and parasitaemia reactivation
After 100th dpi, mice with significantly decreased parasitaemia and established to be in the chronic-phase of the experiment, regardless of the treatment and undetectable by blood microscopy examination, were treated with cyclophosphamide (CP) (ISOPAC) every 4 days by i.p. injection with a dose of 200 mg·kg−1 for a maximum of three doses (Franciscoet al. , 2016) (Scheme 2 ). Within 1 week after the last CP injection, parasitaemia was evaluated as stated above (Scheme 2 ).