Limitations
The histologic examination could not be done. Paraffin chicken fixation (60ÂșC) cooked (turned white) the flesh, resulting in non-differentiation between the lesion sections and raw sections; no volume could be measured. Therefore, it was decided to cut each lesion in the middle and to measure just each lesion surface area. This is an ex-vivo experience, and done in a chicken breast, not myocardium, there is no way to ascertain how the lesions will be formed (and RF delivered) in a beating heart.