Drought tolerance test
Four-month-old plants were used for drought tolerance test in a
greenhouse. We weighed the total weight
of nutrient soil and plant in the
flower pot after watering to the maximum (100%) water holding capacity
(WHC) of soil before drought treatment. Then, control water treatment
was given till WHC was reduced to 50%. The date was recorded as 0 d of
drought treatment. We tested the leaf physiological parameters at 0 d, 7
d, and 10 d after drought treatment. Nine S0-0 and nine S0-50 plants
were randomly selected and divided into three groups as three biological
replicates to evaluate plant drought tolerance. As to the transgenic
plants overexpressing AtSOAR1 , three plants propagated by cuttage
of each line were treated as a biological repeat.