Figure legends
Figure 1.
Timeline: instillations with protamine and CS in the appointed study
group was performed first over the course of 5 hours. The baseline for
TEER measurements was started right afterwards at t=0 hours. At t=7
hours the daily endpoint for recovery was set and evaluated.
Figure 2.
Immunohistochemical staining and Scanning electron microscopy (SEM):
Figure 2A-2C show staining for RGE 53 showing cytokeratin. Figure 2D-2F
show staining for B-catenin which shows the adherence junctions. 2D:
white arrows depicting columnar appearance of signal from the luminal
side down to the basal side of the cell. 2e: dashed white arrow
depicting merely the cell border signal. 2G-2I staining for occluding
showing the tight junctions. 2J-2L: CS56 staining for chondroitin
sulfate with a more diffuse signal with intense cell borders in 2J, less
signal in 2K en more intense signal in 2L. In the negative control group
(2M) cell borders are clearly visible with intact cells. In the
protamine group (2N) the cell borders are still visible, but cracks
start to show. In protamine+CS group (2O) there seems to be a layer
covering the cells.
Figure 3.
TEER measurements on day 1,2,3. Showing a significant increase of TEER
in the protamine + CS group on all 3 days, with also an absolute
increasing value of TEER. In the protamine group, there is a significant
increase of TEER on day 3, but in absolute values TEER resembles damaged
urothelium.