Figure legends
Figure 1.
Timeline: instillations with protamine and CS in the appointed study group was performed first over the course of 5 hours. The baseline for TEER measurements was started right afterwards at t=0 hours. At t=7 hours the daily endpoint for recovery was set and evaluated.
Figure 2.
Immunohistochemical staining and Scanning electron microscopy (SEM): Figure 2A-2C show staining for RGE 53 showing cytokeratin. Figure 2D-2F show staining for B-catenin which shows the adherence junctions. 2D: white arrows depicting columnar appearance of signal from the luminal side down to the basal side of the cell. 2e: dashed white arrow depicting merely the cell border signal. 2G-2I staining for occluding showing the tight junctions. 2J-2L: CS56 staining for chondroitin sulfate with a more diffuse signal with intense cell borders in 2J, less signal in 2K en more intense signal in 2L. In the negative control group (2M) cell borders are clearly visible with intact cells. In the protamine group (2N) the cell borders are still visible, but cracks start to show. In protamine+CS group (2O) there seems to be a layer covering the cells.
Figure 3.
TEER measurements on day 1,2,3. Showing a significant increase of TEER in the protamine + CS group on all 3 days, with also an absolute increasing value of TEER. In the protamine group, there is a significant increase of TEER on day 3, but in absolute values TEER resembles damaged urothelium.