3.5 | DSG inhibited cell survival by inducing UHRF1
protein degradation.
It was reported that UHRF1 depletion remarkably decreased cancer cell
proliferation and viability (Liu, et al., 2020). Since DSG induced UHRF1
protein degradation, we tested the impacts of DSG on PCa cell
proliferation and viability using CCK-8 assays, and calculated the 50%
inhibitory concentrations. DSG inhibited cell proliferation and
viability of PCa cell lines in a dose-dependent manner. More
importantly, the inhibitory effect of DSG on PCa cells is highly and
positively correlated with the expression levels of UHRF1 (Fig. 5a-b).
In the effort to validate whether the inhibitory effect of DSG is
attributed to UHRF1 inhibition, we re-examined the inhibitory effect of
DSG on cell viability of DU145 and PC3 cells when UHRF1 was silenced
with siRNAs. The results showed that UHRF1 knockdown attenuated the
inhibitory effect of DSG (Fig. 5c-e), suggesting that DSG inhibited cell
proliferation and viability of PCa cells by specifically targeting UHRF1
protein.