FIGURE 5. DSG inhibits cell survival by inducing UHRF1 protein
degradation. a PCa cells were treated with different doses of DSG for
48h, and cell survival was analyzed by CCK8 assay. The IC50 values of
DSG were calculated in different cell lines. b Protein levels
of UHRF1 in different PCa cell lines were assessed by western blotting.c PCa cells were transfected with either scramble or UHRF1
siRNAs for 48 h. UHRF1 protein levels were assessed by western blotting.d-e UHRF1-knockdown DU145 and PC3 cells were treated with
different doses of DSG for 48h, and cell survival were analyzed by CCK-8
assay. The error bars represent means ± SDs, n = 5. Significance was
determined by 2-way ANOVA and Tukey’s method, *p < 0.05, **p
< 0.01, ***p < 0.001, and ****p < 0.0001.