FIGURE 6. DSG reduced the genomic DNA methylation
levels, and re-activated the expression of TSGs, resulting in cell cycle
arrest and cellular senescence of PCa cells. a PCa cells were treated
with DSG at different doses for 5 days, and then the genomic DNA was
prepared. The levels of 5mC were assessed by DNA dot blotting, and the
dot intensities were quantified. Significance was determined by
Student’s t-test, *p < 0.05; **p < 0.01, ***p
< 0.001. b DU145 cells were treated with DSG at
different doses for 2 days, and the mRNA levels of TSGs were analyzed by
RT-PCR, β-actin was used as an internal control. Significance was
determined by one-way ANOVA and Tukey’s method, *p < 0.05, **p
< 0.01, ***p < 0.001, and ****p < 0.0001.c DU145 or PC3 cells were treated with DSG for 3 days, and cell
cycle was analyzed by cell cycle analysis kit. Significance was
determined by Student’s t-test, *p < 0.05, **p <
0.01. d DU145 or PC3 cells were treated with DSG for 3 days and
cell senescence was assessed by Senescence β-Galactosidase Staining
assay. Scale bar indicates 100μm.