FIGURE
3. DSG induced UHRF1 protein degradation, but did not change mRNA
levels. a PC3 cells were treated with DSG or L7G at the
indicated doses for 48 h, and the proteins were harvested for
immunoblotting. b PC3 cells were treated with different doses
of DSG or L7G for 48 h. The mRNA levels of UHRF1 were analyzed by
RT-PCR. β-actin was used as an internal control. c C4-2 or
DU145 cells were treated with different doses of DSG for 48 h. Cell
lysates were harvested and the protein levels were assessed by
immunoblotting. d C4-2 or DU145 cells were treated with
different doses of DSG for 48 h. The mRNA levels of UHRF1 were analyzed
by RT-PCR, β-actin was used as an internal control. The statistical
significance was determined by student t-test, ***p < 0.001.