FIGURE 6. DSG reduced the genomic DNA methylation levels, and re-activated the expression of TSGs, resulting in cell cycle arrest and cellular senescence of PCa cells. a PCa cells were treated with DSG at different doses for 5 days, and then the genomic DNA was prepared. The levels of 5mC were assessed by DNA dot blotting, and the dot intensities were quantified. Significance was determined by Student’s t-test, *p < 0.05; **p < 0.01, ***p < 0.001. b DU145 cells were treated with DSG at different doses for 2 days, and the mRNA levels of TSGs were analyzed by RT-PCR, β-actin was used as an internal control. Significance was determined by one-way ANOVA and Tukey’s method, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.c DU145 or PC3 cells were treated with DSG for 3 days, and cell cycle was analyzed by cell cycle analysis kit. Significance was determined by Student’s t-test, *p < 0.05, **p < 0.01. d DU145 or PC3 cells were treated with DSG for 3 days and cell senescence was assessed by Senescence β-Galactosidase Staining assay. Scale bar indicates 100μm.