FIGURE 3. DSG induced UHRF1 protein degradation, but did not change mRNA levels. a PC3 cells were treated with DSG or L7G at the indicated doses for 48 h, and the proteins were harvested for immunoblotting. b PC3 cells were treated with different doses of DSG or L7G for 48 h. The mRNA levels of UHRF1 were analyzed by RT-PCR. β-actin was used as an internal control. c C4-2 or DU145 cells were treated with different doses of DSG for 48 h. Cell lysates were harvested and the protein levels were assessed by immunoblotting. d C4-2 or DU145 cells were treated with different doses of DSG for 48 h. The mRNA levels of UHRF1 were analyzed by RT-PCR, β-actin was used as an internal control. The statistical significance was determined by student t-test, ***p < 0.001.