Figure 1 - Minipool culture in 96 well plates after 5 days. Viable cell density (A), titer (B) and cell normalized titer (C, titer divided by cell density). Red dashed lines indicate 1st and 3rd quartiles and black solid lines indicate medians. While the viable cell density of the Gs/Aspg double KO was much initially lower, the amount of protein produced per cell in minipools was substantially higher for the mutant.