Parasitological examination
Skin samples were collected by scraping with a surgical blade, stored at
4°C and cultured in culture dishes at 37°C for two hours. The scrapings
and cultures from skin samples were smeared on microscope slides with
cedar wood oil and observed using light microscopy. Morphological
characteristics, including the total size; morphology of the idiosoma,
gnathosoma and legs; presence and characteristics of spines on the
dorsal surface; and position of the anal plate, and taxonomic
assignments were recorded(Samuel et al., 2001).