Figure 1. The CRISPR / CAST package detection principle.
Figure 1 shows the CRISPR/CAST package detection principle, and the detection process is divided into three steps. The first step is to use the RPA isothermal amplification technology to amplify theBrucella target DNA; In the second step, the 3 μl RPA amplification products are added to the reaction solution containing Cas12a, crRNA, and the ssDNA with fluorescent report group at 42oC for 10min; The third step is to fill the second reaction solution with pure water to 50 μl and then the test strip inserts into the solution. After the test strip is infiltrated thoroughly, observe the result within 10min with naked eyes. The detection line color is displayed, and the result is positive. The result is negative if the detection line has no color and the quality control line is displayed. The result is invalid if the quality control and detection line color are displayed.