C
Figure 3. Sensitivity of three methods. A. Sensitivity of the standard RPA for nucleic acid detection. After amplification at 39°C for 15min, 5 μl of each reaction was electrophoresed (2% agarose), M: 500 bp Marker (Takara, Code No.3590Q), 1-8 corresponds to the copies of plasmid standard were 108-100copies/μl, N: a negative control. B. Sensitivity of the DETECTR method. A volume of 3 μl of standard RPA product was added to the Cas12a cleavage system, and maximum fluorescent signals were recorded (n = 3, error bars showed mean ± SEM). C. Sensitivity of the CRISPR/CAST package, 1-8 corresponding to the plasmid copy number is 107-100 copies/μl, N: negative control, the green arrow was the quality control line, and the orange arrow was the detection line.