1.3.6 CRISPR/CAST package specificity test
Compare primers and crRNA sequences with Brucella (GenBank No.
CP025819, CP025820) and its cross-strains (Yersinia
enterocolitica O:9, Escherichia coli O157, Salmonella
enterica serovar Urbana O:30, and Francisella tularensis )
by BLAST function. Using the CRISPR/CAST package method as described in
1.3.3. Simultaneous assay of Brucella , Yersinia
enterocolitica O:9, Escherichia coli O157, Salmonella
enterica serovar Urbana O:30, and Francisella tularensis ,
RNase-free water as a negative control for specificity test.