C
Figure 3. Sensitivity of three methods. A. Sensitivity of the
standard RPA for nucleic acid detection. After amplification at 39°C for
15min, 5 μl of each reaction was electrophoresed (2% agarose), M: 500
bp Marker (Takara, Code No.3590Q), 1-8 corresponds to the copies of
plasmid standard were 108-100copies/μl, N: a negative control. B. Sensitivity of the DETECTR method.
A volume of 3 μl of standard RPA product was added to the Cas12a
cleavage system, and maximum fluorescent signals were recorded (n = 3,
error bars showed mean ± SEM). C. Sensitivity of the CRISPR/CAST
package, 1-8 corresponding to the plasmid copy number is
107-100 copies/μl, N: negative
control, the green arrow was the quality control line, and the orange
arrow was the detection line.