2.2 CRISPR/CAST package sensitivity test
The RPA sensitivity test that prepared a series of diluted positive
quality controls with the number of copies was
108−100 copies/μl as the template
and RNase-free water as the negative control. The CRISPR/CAST package
sensitivity test that prepared a series of diluted positive quality
controls with the number of copies was
107−100 copies/μl as the template
and RNase-free water as the negative control. The results are exhibited
in Figure 3. The lowerdetection limit for RPA is 1000 copies/μl (Figure
3A), and the CRISPR/CAST package sensitivity test is 10 copies/ μl
(Figure 3B, 3C).