2.4 Effect evaluation of the serum samples test
The blood samples of 398 sheep and 100 cattle from the Tongliao Zarut Banner area were tested by RBT. Through extracting DNA of serum samples, then adopting the CRISPR/CAST package method as mentioned in 1.3.3 and the qPCR to assay Brucella DNA, the results in Table 3, of which 31 sheep and 8 cattle were Brucella DNA positive. The detection rate was consistent with the qPCR and higher than RBT’s (19 sheep and 5 cattle serum were positive).