2.2 CRISPR/CAST package sensitivity test
The RPA sensitivity test that prepared a series of diluted positive quality controls with the number of copies was 108−100 copies/μl as the template and RNase-free water as the negative control. The CRISPR/CAST package sensitivity test that prepared a series of diluted positive quality controls with the number of copies was 107−100 copies/μl as the template and RNase-free water as the negative control. The results are exhibited in Figure 3. The lowerdetection limit for RPA is 1000 copies/μl (Figure 3A), and the CRISPR/CAST package sensitivity test is 10 copies/ μl (Figure 3B, 3C).