1.3.6 CRISPR/CAST package specificity test
Compare primers and crRNA sequences with Brucella (GenBank No. CP025819, CP025820) and its cross-strains (Yersinia enterocolitica O:9, Escherichia coli O157, Salmonella enterica serovar Urbana O:30, and Francisella tularensis ) by BLAST function. Using the CRISPR/CAST package method as described in 1.3.3. Simultaneous assay of Brucella , Yersinia enterocolitica O:9, Escherichia coli O157, Salmonella enterica serovar Urbana O:30, and Francisella tularensis , RNase-free water as a negative control for specificity test.