Figure 1. The CRISPR / CAST package detection principle.
Figure 1 shows the CRISPR/CAST package detection principle, and the
detection process is divided into three steps. The first step is to use
the RPA isothermal amplification technology to amplify theBrucella target DNA; In the second step, the 3 μl RPA
amplification products are added to the reaction solution containing
Cas12a, crRNA, and the ssDNA with fluorescent report group at
42oC for 10min; The third step is to fill the second
reaction solution with pure water to 50 μl and then the test strip
inserts into the solution. After the test strip is infiltrated
thoroughly, observe the result within 10min with naked eyes. The
detection line color is displayed, and the result is positive. The
result is negative if the detection line has no color and the quality
control line is displayed. The result is invalid if the quality control
and detection line color are displayed.