1.3.2 crRNAs and probes design
According to the LbCas12a protein corresponding to the crRNA stem-loop
structure and the target gene sequence, referring to the crRNA design
requirements (Zhu & Liang, 2019), four groups of specific crRNA (Table
2) were designed on the IS711 gene sequence of Brucella. We
referred to Chen et al for the principles of reporter probe design (Chen
et al., 2018). The fluorescent probe (yg-probe) sequence is
6-FAM-TTATT-BHQ. The test strip probe refers to the principal design of
nucleic acid test strip detection, the sequencing probe (szt-probe) is
6-FAM-TTATT-Biotin. crRNAs and probes were synthesized by Sangon Biotech
(Shanghai, China).