Authors:
Xiaojun Yin1,†, Carmen H. Li1,2,†(https://orcid.org/0000-0002-6135-2582), Alper Celik3, Julia E. M. Upton4, 5(https://orcid.org/0000-0001-5320-4232), Christina M. Ditlof1, 6Ditlof1,6(https://orcid.org/0000-0003-3253-4050), Jennifer A. Hoang1, 6(https://orcid.org/0000-0001-5320-4232), Alireza Berenjy1, Akash Kothari1, 2(https://orcid.org/0000-0003-1980-161X), Lisa Hung1,6 (https://orcid.org/0000-0002-4590-1895), Thomas Eiwegger1,6,7,8(https://orcid.org/0000-0002-2914-7829)
Affiliations :
1Translational Medicine Program, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
3Centre for Computational Medicine, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
4Department of Pediatrics, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
5Division of Immunology and Allergy, SickKids Food Allergy and Anaphylaxis Program, Hospital for Sick Children
6Department of Immunology, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
7Karl Landsteiner University of Health Sciences, Krems an der Donau, Austria,
8Department of Pediatric and Adolescent Medicine, University Hospital St. Pölten, St. Pölten, Austria
Contributed equally
Corresponding Author : Thomas Eiwegger, MD, Department of Pediatric and Adolescent Medicine, University Hospital St. Pölten, Dunant-Platz 1, 3100 St. Pölten, Austria. E-mail: thomas.eiwegger@stpoelten.lknoe.at, Tel.: +43 2742-9004-11740
Conflict of Interest Statement: XY, CHL, AC, CMD, JAH, AB, AK, and LH have nothing to disclose. JEMU reports grants/research support from DBV Technologies, Regeneron, CIHR, ALK-Abello, SickKids Food Allergy and Anaphylaxis Program, Advisory board for Pfizer, Kaleo, Bausch Health, Food Allergy Canada; in-kind drug donation from Novartis, other for Astra-Zeneca, all outside the current work. TE reports to act as local PI for company sponsored trials by DBV and sub-investigator for Regeneron, holds grants from Innovation Fund Denmark, CIHR outside the submitted work. He is Co-Investigator or scientific lead in three investigator-initiated oral immunotherapy trials supported by the Food Allergy and Anaphylaxis Program SickKids and serves as an associate editor for Allergy. He/his lab received unconditional/in-kind contributions from Macro Array Diagnostics and an unrestricted grant from ALK. He holds advisory board roles for ALK, Nutricia/Danone and Aimmune. TE reports lecture fees from Novartis.
Funding Information: This work was supported by The Hospital for Sick Children (Food Allergy and Anaphylaxis Program, start-up funds by the SickKids Research Institute and the Department of Pediatrics, Restracomp Graduate Scholarship to CHL, AK, and LH). CHL, AK, LH are the recipients of the Canadian Institutes of Health Research Fredrick Banting and Charles Best Canada Graduate Scholarship.
Statement of Author Contribution: XY, CHL, AC, CMD, JAH, and AB contributed to data acquisition. JEMU and TE contributed to experimental designs. XY, CHL, AC, AK, LH, JEMU, and TE contributed to data interpretation and preparation of the manuscript. The final version of the manuscript was approved by all authors.
Keywords : food allergy, pediatrics, mass cytometry
Abbreviations : CyTOF; cytometry by time-of-flight, OIT; oral immunotherapy, PMA/Iono; Phorbol 12-myristate 13-acetate-ionomycin, tSNE; t-Distributed Stochastic Neighbour Embedding
Word Count: 589/600