Passive sensitization
PCIS were passively sensitized in a 1:10 dilution of human plasma from
clinically confirmed peanut allergic donors or peanut-sensitized
non-allergic donors (Table S2) in WME for 90-120min
(37oC, 90% O2, 5%
CO2) with gentle shaking. Human plasma was derived from
ongoing and completed trials including the Markers of Nut Allergy Study
(MONAS) cohort at the Hospital for Sick Children.27The use of human plasma for research was approved by the SickKids REB
for MONAS (#1000053791) and the Low Dose Multi-OIT (LoMo) study
(#1000060633).