scNucSeq of gut tissue confirms the presence of mast cells and
other cell types
The cellular composition of PCIS
was analyzed using scNucSeq. Single cell gene expression profiles from
colon tissue donors (n=4) were compiled to reveal the cellular
composition of PCIS (Figure 1D). Expression profiles aligned with the
cellular identities of each cluster and were confirmed using single cell
gene expression databases.25,26 All expected cell
types in colon tissue were observed (Figure 1D, Table S4) including
epithelial cells (enterocytes, goblet cells, enteroendocrine cells,
endothelial cells), stromal cells (fibroblasts, smooth muscle cells) and
immune cells (T cells, γδT cells, B cells, mast cells, dendritic cells,
macrophages).