Figure 5. Real-time PCR analysis results showed that treatment with rh-EGF protein results in an over-expression of PDGF, VEGF, and TGF-β genes. A, B, and C diagrams are related to PDGF, VEGF, and TGF-β gene expressions respectively. The results show no significant changes in the expression level of the aforementioned gene treated with free chitosan. When treated with EGF-CS NPs, a higher expression level of the three intended genes has been observed.