Plasmid hydrodynamic injection and acute hepatic failure model.
100 μg plasmid in 2 ml PBS solution was injected into the tail vein of mice within 8-12 seconds. 8-week-old C57BL/6 mice were intraperitoneally injected with LPS/D-GalN at 10 μL/g body weight (D-GalN: 200 mg/kg body weight. LPS: 10 μg/kg body weight) respectively. After injection, the mice were kept in a specific pathogen free (SPF) room, and the survival status of mice was recorded in 24 hours.