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\begin{document}

\title{KIR2DL4/HLA-G polymorphisms were associated with HCV infection
susceptibility among Chinese high-risk population}



\author[1]{Ming Yue}%
\author[2]{Zepei Feng}%
\author[2]{Peng Huang}%
\author[3]{Jinwei Zhang}%
\author[4]{Xueshan Xia}%
\author[4]{A-Mei Zhang}%
\author[1]{Tian Zeng}%
\author[5]{Qiong Chen}%
\author[1]{Chuanlong Zhu}%
\author[5]{Wei-long Tan}%
\author[6]{Yun Zhang}%
\affil[1]{Affiliated Hospital of Nanjing Medical University}%
\affil[2]{Nanjing Medical University Department of Epidemiology}%
\affil[3]{Affiliated Drum Tower Hospital of Medical College of Nanjing University}%
\affil[4]{Kunming University of Science and Technology}%
\affil[5]{Eastern Theater Command Centers for Disease Prevention and Control}%
\affil[6]{Nanjing Medical University Center for Global Health}%


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  \date{June 05, 2024}


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\begin{abstract}
\textbf{Background:} Killer-cell immunoglobulin-like receptors 2DL4 (
\emph{KIR2DL4}) and the human leukocyte antigen class I-G (
\emph{HLA-G}) display vital parts in immune responses against HCV
infection. We select four potentially functional SNPs of KIR/HLA to
explore the associations between \emph{KIR2DL4/HLA-G} genetic variants
and HCV infection results. \textbf{Methods:} In the present case-control
investigation, \emph{KIR2DL4}-rs660773, \emph{KIR2DL4}-rs660437,
\emph{HLA-G}-rs9380142, and \emph{HLA-G}-rs1707 SNPs were sorted as
genotype in a total of 2225 high-risk subjects, involving 1778 paid
blood donors and 447 drug users. The SNPs were functionally annotated
using bioinformatics analysis. \textbf{Results:} Following adjusting by
age, sex, ALT, AST, \emph{IFNL4}-rs12979860, \emph{IFNL4}-rs8099917, and
the infection route, the logistic regression analysis (LRA) discovered
that \emph{KIR2DL4}-rs660773 and \emph{HLA-G}-rs9380142 were correlated
with vulnerability to HCV infection (all \emph{P} \textless{}0.05). In a
locus-dosage way, compared with subjects carrying the rs9380142-AA or
rs660773-AA genotypes, subjects with rs9380142-AG or rs660773-AG/GG (all
\emph{P} \textless{}0.05) were more vulnerable to HCV infection; the
overall impact of their risk genotypes (rs9380142-AGrs660773-AG/GG) was
correlated with an elevated incidence of HCV infection (
\emph{P\textsubscript{trend}}\textless{}0.001). In the Haplotype
analysis, patients with haplotype AG were more likely to contract HCV
compared to those with the highest common AA haplotype ( \emph{P}=
0.002) were higher in susceptibility to infect HCV.nThe SNPinfo web
server estimated that rs660773 is a transcription factor binding site
(TFBS), whereas rs9380142 is a potential microRNA-binding site.
\textbf{Conclusion:} In the Chinese high-risk population, KIR2DL4
rs660773 and HLA-G rs9380142 polymorphisms are related to HCV
susceptibility \emph{.}%
\end{abstract}%



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