Figure 4. Profiles of differentially expressed transcripts demonstrate opposing responses in Bt-infected individuals that were or were not also exposed to or selected with pesticides. Transcripts significantly differentially expressed (padj < 0.05) with Bt treatment and interactions with A. OP exposure, and B.Pyr selection regime and exposure. The DE Factor/ Model legend refers to the factor corresponding to the differentially expressed gene set and the DESeq2 model used (no pesticide- hatched bars; OP or Pyr- solid bars; BtTx = main effect of Btt treatment; OP PTx = main effect of OP exposure; OP or Pyr PTx:BtTx = interactions between OP or Pyr exposure and Btt treatment; Pyr Reg:BtTx: interaction between Pyr selection regime and Btt treatment). Colored circles on the left indicate transcripts included in the magenta WGCNA module (Suppl. Fig. 7). Transcript abbreviations are as follows: Stc = shuttle craft-like protein, Def = defensin, Att = attacin, PR = pathogenesis-related protein, HRG = histidine-rich glycoprotein, SP = serine protease, P450 = cytochrome P450, CEH = carboxylic ester hydrolase, GST = glutathione S-transferase, ABC = ABC transporter, Chit = chitinase protein, CP = cuticle-related protein, PM = peritrophic matrix related protein, Vit = vitellogenin, Ins = insulin-like peptide, LSP = larval serum protein, AchE = acetylcholinesterase, KLF = Krüppel-like factor, ApoIII = apolipophorin-III, Neur = neuropeptide.