Figure 4. Profiles of differentially expressed transcripts
demonstrate opposing responses in Bt-infected individuals that were or
were not also exposed to or selected with pesticides. Transcripts
significantly differentially expressed (padj < 0.05) with Bt
treatment and interactions with A. OP exposure, and B.Pyr selection regime and exposure. The DE Factor/ Model legend refers to
the factor corresponding to the differentially expressed gene set and
the DESeq2 model used (no pesticide- hatched bars; OP or Pyr- solid
bars; BtTx = main effect of Btt treatment; OP PTx = main effect
of OP exposure; OP or Pyr PTx:BtTx = interactions between OP or Pyr
exposure and Btt treatment; Pyr Reg:BtTx: interaction between Pyr
selection regime and Btt treatment). Colored circles on the left
indicate transcripts included in the magenta WGCNA module (Suppl. Fig.
7). Transcript abbreviations are as follows: Stc = shuttle craft-like
protein, Def = defensin, Att = attacin, PR = pathogenesis-related
protein, HRG = histidine-rich glycoprotein, SP = serine protease, P450 =
cytochrome P450, CEH = carboxylic ester hydrolase, GST = glutathione
S-transferase, ABC = ABC transporter, Chit = chitinase protein, CP =
cuticle-related protein, PM = peritrophic matrix related protein, Vit =
vitellogenin, Ins = insulin-like peptide, LSP = larval serum protein,
AchE = acetylcholinesterase, KLF = Krüppel-like factor, ApoIII =
apolipophorin-III, Neur = neuropeptide.