Figure 3. Number of significantly differentially expressed (sig. DE) genes (padj < 0.05) for factors within each DESeq2 model (number of sig. DE transcripts are denoted above bars; relative to control regime or no pesticide/ Bt treatments, positive counts are upregulated transcripts and negative counts are downregulated). A. No pesticide model: main and interaction effects of pesticide selection regime and Bt treatment in the absence of pesticide exposure. Hatched bars indicate no pesticide treatment in the model. B. OP model: main and interaction effects of OP selection regime, pesticide exposure, and Bt treatment. C. Pyr model: main and interaction effects of Pyr selection regime, pesticide exposure, and Bt treatment. The table below indicates the differentially expressed factor. Main effect of selection regimes = Regime OP/ Pyr. Main effect of Bt treatment = Bt Tx +. Main effect of pesticide treatments = Pesticide Tx OP/ Pyr. Interactions between selection regimes and pesticide exposure = Regime OP/ Pyr & Pesticide Tx OP/ Pyr. Interactions between selection regimes and Bt exposure = Regime OP/ Pyr & Bt Tx +. Interactions between pesticide and Bt exposure = Pesticide Tx OP/ Pyr & Bt Tx +.