Identification of sucrose synthase from Micractinium conductrix to favor biocatalytic glycosylation
Lei Lin§, Ruiqi Ma§, Kai Chen, Jiajie Ding, Huayi Pan, Yehui Tao, Yan Li*, Honghua Jia
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, P. R. China
Keywords: Sucrose synthase, Glycosyltransferase, Glycosylation, Ginsenoside Rh2
* Corresponding author.
Yan Li
Email address:liyan@njtech.edu.cn
ORCID iD:https://orcid.org/0000-0002-5574-2314
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University
Address: No.30, South Puzhu Road, Nanjing 211816, P. R. China
§ These authors contributed equally to this work.
Abbreviations: ADP , adenosine 5’-diphosphate; HEPES , N-2-hydroxyethylpiperazine-N-ethane-sulphonicacid; SuSy , Sucrose synthase; UDP , uridine 5’-diphosphate; UDPG , uridine 5’-diphosphate glucose; UGT , UDP-glycosyltransferase