Figure 5. Effect of medium supplementation on the production of AAV6. HyCell TransFx-H (basal medium) was supplemented with glucose (7.2 g/L final concentration) or with 15% Cell Boost 5. A) Cell growth kinetics and viability. Black arrows indicate the time of transfection. B) Transfection efficiency is described as the percentage of cells expressing the transgene (GFP) 24 hours post-transfection (hpt). C) Volumetric (VG/mL) and cell-specific viral yields (VG/cell) of low cell-density control and high cell-density productions with basal and supplemented media. Values represent mean ± standard deviation (n=3).