Plasmids
The following plasmids were used to produce adeno-associated viral vectors: (1) pAdDeltaF6 was a gift from James M. Wilson (Addgene plasmid #112867; http://n2t.net/addgene:112867; RRID:Addgene_112867), (2) pRep2Cap6 (provided by Dr. Samulski, University of North Carolina, USA), (3) pAAV-CAG-GFP was a gift from Edward Boyden (Addgene plasmid #37825; http://n2t.net/addgene:37825; RRID:Addgene_37825) and (4) pX601-AAV-saCas9-sgRNA, modified from pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA, a gift from Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID:Addgene_61591).