Figure 7. Bioreactor production of AAV6 at medium cell density.Cells were transfected at 4 million cells per mL (medium cell density,
MCD), and plasmid DNA was delivered on a cell basis (1
µg/106 cells). Low-cell-density (LCD, 1 ×
106 cell/mL) bioreactor productions shown as control.
Time indicated as hours post-transfection (hpt). A) Bioreactor control
chart. B) Cell growth kinetics and viability. C) Volumetric titer
(VG/mL). D) Cell-specific viral yield (VG/cell). E) Functional titer
(ETU/mL). F) Ratio between volumetric and functional titer (VG/ETU).
Values represent mean ± standard deviation (n=1 for MCD; n=2 for LCD).