Figure 7. Bioreactor production of AAV6 at medium cell density.Cells were transfected at 4 million cells per mL (medium cell density, MCD), and plasmid DNA was delivered on a cell basis (1 µg/106 cells). Low-cell-density (LCD, 1 × 106 cell/mL) bioreactor productions shown as control. Time indicated as hours post-transfection (hpt). A) Bioreactor control chart. B) Cell growth kinetics and viability. C) Volumetric titer (VG/mL). D) Cell-specific viral yield (VG/cell). E) Functional titer (ETU/mL). F) Ratio between volumetric and functional titer (VG/ETU). Values represent mean ± standard deviation (n=1 for MCD; n=2 for LCD).