Figure 4. Metabolic profile of production of AAV6 via triple transient transfection at high cell density. Cells were transfected at low cell density (LCD – 1 × 106 cells/mL) as control or at high cell density (HCD – 10 × 106 cells/mL). Plasmid DNA was delivered on a cell basis (1 µg/106cells). A) Nutrient and metabolite concentration for production at low and high cell densities using basal or supplemented medium (7.2 g/L final glucose concentration or 15% Cell Boost 5). The dashed line represents the time of transfection. B) Concentration of glucose for productions at low and high cell densities. Time expressed as hours post-transfection (hpt). Values represent mean ± standard deviation (n=3).