Determination of transfection efficiency
For evaluation of transfection efficiency, cells were collected 24 hours
post-transduction and fixed with a solution of 2% paraformaldehyde.
Cells were analyzed using a BD Accuri C6 flow cytometer (BD Biosciences,
USA), and EGFP expression was analyzed using BD Accuri C6 Plus Analysis
Software (BD Biosciences, USA).