Figure 3. Production of AAV6 via triple transient transfection at high cell density. A) transfection efficiency is described as the percentage of cells expressing the transgene (GFP) 24 hours post-transfection. B) Cell growth kinetics (●) and volumetric viral yield (□). Cells were transfected at low cell density (LCD – 1 × 106 cells/mL) as control or at high cell density (HCD – 10 × 106 cells/mL). Plasmid DNA was delivered on a cell basis (1 µg/106 cells). Values represent mean ± standard deviation (n=3). *** P < 0.001, by Student’s T test.