Figure 4. Metabolic profile of production of AAV6 via triple
transient transfection at high cell density. Cells were transfected at
low cell density (LCD – 1 × 106 cells/mL) as control
or at high cell density (HCD – 10 × 106 cells/mL).
Plasmid DNA was delivered on a cell basis (1 µg/106cells). A) Nutrient and metabolite concentration for production at low
and high cell densities using basal or supplemented medium (7.2 g/L
final glucose concentration or 15% Cell Boost 5). The dashed line
represents the time of transfection. B) Concentration of glucose for
productions at low and high cell densities. Time expressed as hours
post-transfection (hpt). Values represent mean ± standard deviation
(n=3).