Plasmids
The following plasmids were used to produce adeno-associated viral
vectors: (1) pAdDeltaF6 was a gift from James M. Wilson (Addgene plasmid
#112867; http://n2t.net/addgene:112867; RRID:Addgene_112867), (2)
pRep2Cap6 (provided by Dr. Samulski, University of North Carolina, USA),
(3) pAAV-CAG-GFP was a gift from Edward Boyden (Addgene plasmid #37825;
http://n2t.net/addgene:37825; RRID:Addgene_37825) and (4)
pX601-AAV-saCas9-sgRNA, modified from
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA, a gift from
Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591;
RRID:Addgene_61591).