High-cell-density production of adeno-associated viral vector
serotype 6 by triple transfection in suspension HEK293 cell cultures
Pablo D. Moço1
Xingge Xu1
Amine A. Kamen1
1Department of Bioengineering, McGill University,
Montreal, Canada
Correspondence: Dr. Amine Kamen, Department of Bioengineering,
McGill University, 3480 University Street | McConnell
Engineering Building, Room 363, Montreal, QC, H3A 0E9 Canada.
E-mail : amine.kamen@mcgill.ca
Keywords: Bioprocess engineering, Bioprocess development, Cell
culture, Mammalian cells, Viruses.
Abbreviations: AAV , adeno-associated virus; AAV6 , AAV
serotype 6; CDE , cell density effect; CSVY ,
cell-specific viral yield; ETU , enhanced transducing units;FDA , U. S. Food and Drug Administration; GFP , green
fluorescent protein; GOI , gene of interest; HCD , high
cell density; LCD , low cell density, MCD , medium cell
density; PEI , polyethylenimine; VG , viral genomes.