Radical scavenging activities of active fractions
The ethanol extracts of RG, CG, and MCG were patriated by different
polarity solutions involving hexane,
chloroform,
ethyl acetate,n -butanol and residual
water-layer extracts. Fifteen fractions were obtained. A total of 48
CAEs were collected from sixteen kinds of enzyme-assisted ginseng
residues.
All fifteen ethanol extracts showed
low scavenging activities. Among the 48 CAEs, the scavenging capability
of OH- in the three ginsengs showed stronger
activities than those of DPPH and O2-. Among the
sixteen kinds of enzymes, alkaline protease-assisted extract of MCG
(APMCG) demonstrated the highest inhibitory effect on
OH- in a dose-dependent manner (Fig. 1A, 1B, 1C). Four
active factions were harvested from the step-gradient ethanol
precipitation at ratios of 30%, 60%, 80%, and 90%, and they named
APMCG-1, APMCG-2, APMCG-3, and APMCG-4, respectively. As shown in
Fig.1D, APMCG-1 demonstrated the most marked inhibitory effect on
OH- (84.16%).