Cytotoxicity and ROS production
The cytotoxicity of the extracts was determined via MTT assay, and
intercellular ROS production was evaluated by
DCFH-DA probe after
PA explosion (Dai, Jiang, Lu, et
al., 2020). Two cells in the PA group were added with 0.1, 0.2, 0.3, 0.4
and 0.5 mM PA to screen for the appropriate stimulating dose. The cells
were seeded at a density of 1×106 cells/mL in a
96-well plate for 24 h and treated with the indicated concentrations of
PA. The untreated cells were regarded as blank. After 24 h incubation,
50 μL of 2 mg/mL MTT solution was added to the cells. After another 3 h,
the supernatant was discarded, and 200 μL of dimethyl sulfoxide was
added to each well. The absorbance at 540 nm was determined by
enzyme-linked immunosorbent assay to calculate the cell viability.
Similarly, the cytotoxicity and ROS production of samples in PA-induced
cells were determined by MTT and DCFH-DA assays, respectively.