Protective effect of extracts on adult zebrafish
To further confirm the protective effect of extracts, we treated
1-month-old zebrafish (designed as ”0 week”) with a 1.75% glucose and
10% cholesterol diet for 4 weeks to establish a type 2 diabetic
zebrafish model. The sample treatment group was given the indicated
concentrations of extracts from 5th week to 8th week. The control group
was fed a normal commercial diet. Zebrafish in each group were fed twice
a day, and the medium was replaced with a fresh one group. After fasting
for 12 h on the last day of the 8th week, the body weight of the
zebrafish was measured. The muscular endurance of ginseng-treated
zebrafish was evaluated following a previous method (Kim et al., 2019).
The zebrafish were euthanized by MS 222, and muscle tissue was collected
for analysis. The levels of FBG,
INS, TG, T-CHO, FFA,
MG, SOD, GSH-PX, CAT, MDA, TNF-ɑ
and IL-6 were calculated following kit instructions.
In addition, the heart and muscle tissues of zebrafish in each group
were collected and treated with 4% paraformaldehyde for 48 h. The
hematoxylin-eosin (HE), TUNEL assay and DAPI staining, and
immunohistochemistry (IHC) staining were performed by previous protocols
(Li et al., 2018).