Evaluation of mitochondrial membrane potential (MMP)
Rhodamine 123 probe was used for MMP evaluation. Briefly, cardiomyocytes and differentiated skeletal myoblasts were treated with PA and/or samples for 24 h incubation. The medium was discarded and serum-free culture medium containing 0.1 μg/mL rhodamine 123 was used to mount cells for 45 min incubation at 37 °C. The cells were observed under a fluorescence microscope (Olympus CKX53, Tokyo, Japan).