Hoechst 33342 and PI fluorescent staining assay
The cardiomyocytes and differentiated skeletal myoblasts were,
respectively, treated with PA and/or samples in 6-well plates. PBS was
used to wash the cells twice and substituted in the cell medium. Then,
PBS was discarded, and Hoechst
33342 and PI were added to the cells for 20 min incubation in the dark.
The cells were visualized by a fluorescent microscope (Olympus CKX53,
Tokyo, Japan).