Determination of intracellular Ca2+ levels
In accordance with the instructions of commercial kits, the productions
of Ca2+ in cardiomyocytes and differentiated skeletal
myoblasts treated with PA and/or samples were evaluated with a
fluo-8AM probe. The cell medium
was discarded, and PBS solution containing 4 μM of fluo-8AM was added to
the cells for 45 min incubation. Calcium production in each group was
imaged by a fluorescent microscope (Olympus CKX53, Tokyo, Japan).