Determinations of CK and LDH
To understand the protective effects of extracts on PA-induced cardiomyocytes and skeletal myoblasts, we evaluated the contents of CK and LDH in the two cell lines using commercial kits. Briefly, the cells were treated with PA and/or samples for 24 h. The contents of CK and LDH were determined by measuring the absorbance at 660 and 440 nm, respectively.