Protective effect of extracts in larvae
The Tg (kdrl:EGFP) zebrafish (5 days post fertilization) were used to model the simulation of T2DM symptoms. The zebrafish were given 10% high-fat diet for 8 h and 3% glucose solution for 16 h in a day (every 24 h) (Wang et al., 2013). The feeding scheme was followed consecutively for 4 days, and the type 2 diabetic zebrafish model was established. The control group was given a normal commercial diet. In the sample-treated group, the zebrafish were administrated with a normal commercial diet, and the sample was dissolved in the medium. The medium in each well was replaced with a fresh one every 24 h.
The heart rate of zebrafish strongly influences the functional metabolism of their cardiomyocytes and skeletal myoblasts. Therefore, the indicated factors were recorded. In order to further verify whether the reduction of vascular lipid accumulation in type 2 diabetic patients with APMCG-1 is related to blood flow velocity, we treated 5dpf AB zebrafish with the modeling method described above. The Micro Zebra Lab application (view version 3.4.4, Lyon, France) is used to detect blood flow velocity in major blood vessels (Benslimane et al., 2020).
In accordance with the instructions of commercial kits, the contents of fasting blood glucose (FBG), TG, T-CHO, SOD, GSH-PX, CAT, MDA, TNF-ɑ, IL-6, CK-MB, and cardiac troponin I cTnI were measured.