Determinations of CK and LDH
To understand the protective effects of extracts on PA-induced
cardiomyocytes and skeletal myoblasts, we evaluated the contents of CK
and LDH in the two cell lines using commercial kits. Briefly, the cells
were treated with PA and/or samples for 24 h. The contents of CK and LDH
were determined by measuring the absorbance at 660 and 440 nm,
respectively.