Cell culture
H9c2(2-1) cardiomyocytes and C2C12 skeletal muscle-derived myoblasts were purchased from Wuhan Pnuosai Life Technology Co., LTD and incubated in 5% carbon dioxide at 37 °C in a constant temperature incubator. H9c2 and C2C12 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (high-glucose DMEM medium + 10% fetal bovine serum +1% P/S). The differentiation medium (high-glucose DMEM medium + 2% horse serum +1% P/S) was used to differentiate C2C12 cells over a period of 5-7 days. Differentiation was confirmed by visualization of the formation of multinucleated myotubes.