Cell culture
H9c2(2-1) cardiomyocytes and C2C12 skeletal muscle-derived myoblasts
were purchased from Wuhan Pnuosai Life Technology Co., LTD and incubated
in 5% carbon dioxide at 37 °C in a constant temperature incubator. H9c2
and C2C12 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)
(high-glucose DMEM medium + 10% fetal bovine serum +1% P/S). The
differentiation medium (high-glucose DMEM medium + 2% horse serum +1%
P/S) was used to differentiate C2C12 cells over a period of 5-7 days.
Differentiation was confirmed by visualization of the formation of
multinucleated myotubes.