Evaluation of mitochondrial membrane potential (MMP)
Rhodamine 123 probe was used for
MMP evaluation. Briefly, cardiomyocytes and differentiated skeletal
myoblasts were treated with PA and/or samples for 24 h incubation. The
medium was discarded and serum-free culture medium containing 0.1 μg/mL
rhodamine 123 was used to mount cells for 45 min incubation at 37 °C.
The cells were observed under a fluorescence microscope (Olympus CKX53,
Tokyo, Japan).