Figure 4 . Chromatograms from AEX processes employing the
following: (A–B) an isocratic wash containing
20 mM BTP, 0.001% (w/v) Pluronic
F-68, and 206.5 mM TEA-Ac at pH 9 over 10 CVs, and a LGE containing
20 mM BTP, 0.001% (w/v) Pluronic
F-68, 2 mM MgCl2, and 25–182.5 mM NaCl at pH 9 over 30
CVs; (C–D) an isocratic wash containing 20 mM BTP, 0.001% (w/v)
Pluronic F-68, 206.5 mM TEA-Ac at pH 9 over 10 CVs, a second isocratic
wash containing 20 mM BTP, 0.001% (w/v) Pluronic F-68, 2 mM
MgCl2, and 25 mM NaCl at pH 9 over 10 CVs, and an
isocratic elution containing 20 mM
BTP, 0.001% (w/v) Pluronic F-68, 2 mM MgCl2, 116.7 mM
NaCl at pH 9 over 10 CVs. (B) and (D) are magnified images of empty
(left panel) and full-capsid peaks (right panel) from chromatograms (A)
and (C), respectively. Blue and red traces correspond to
A280 and A254, respectively. The dotted
purple traces and dashed green traces correspond to the volumetric
fraction of running buffer B pumped through the system and the solution
conductivity, respectively.