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\begin{document}

\title{Dual-slit confocal light-sheet microscopy using birefringent crystals}



\author[1]{Xin Xu}%
\author[2]{Hone Ye}%
\author[1]{Jixiang Wang}%
\author[2]{Xin Zhang}%
\author[1]{Guohua Shi}%
\affil[1]{University of Science and Technology of China}%
\affil[2]{Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Science}%


\vspace{-1em}



  
  \date{June 10, 2024}


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\begin{abstract}
We present a method to use a birefringent crystal for generating two
illumination beams in a digital scanned laser light-sheet microscopy
(DSLM) system. Upon this, a conventional confocal DSLM can be easily
upgraded to a dual-slit confocal DSLM with two-fold imaging speed. We
have implemented this method to our bidirectional DSLM system, locating
two identical calcite crystals on both illumination paths from both
sides of the sample. The neurons of \emph{in vivo} larval zebrafish have
been fast imaged with sterling image quality, especially
\textasciitilde{}2.5 times higher contrast, compared to the conventional
DSLM.%
\end{abstract}%



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\textbf{Hosted file}

\verb`Dual-slit confocal light-sheet microscopy using birefringent crystals.docx`  available at \url{https://authorea.com/users/586435/articles/624576-dual-slit-confocal-light-sheet-microscopy-using-birefringent-crystals}

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