FIGURE 4  Macrophages polarized in response to DP scaffold promote BMSC migration and differentiation in vitro.
The viability of BMSCs after culture in different types of conditioned medium (CM) for 1 and 3 days. (B&C) Representative images and quantitative analysis of BMSC migration after culture in different CM for 1 day. Scale bar, 50 μm. (D&E) Transwell-based BMSC migration assays and quantification analysis. Scale bar, 1 mm.(F) ARS staining on the 21th day in culture. (G) Quantitative results of ARS staining on the 21th day culture. (H) mRNA levels ofRunx2 and BMP2 in BMSCs after culture in different CM as quantified by qRT-PCR.