FIGURE 3  DP scaffolds regulate macrophage polarization in vitro.
(A) Viability of BMSCs seeded on each scaffold type at the indicated time points. (B) Viability of RAW264.7 cells seeded on each scaffold type at the indicated time points. (C) Flow cytometric analysis of macrophage population expressing CD86 (M1 type) and CD206 (M2 type) at 3 days after seeding. (D) mRNA levels of Arg-1 and iNOS in macrophages on the 3rd day in culture determined by qRT-PCR. (E) IL-10 and TNF-α levels in the supernatants on the 3rd day in culture as detected by ELISA. (F) Heatmap of the transcriptomes of RAW264.7 cells after 3 days in culture on COL and DP scaffolds. (G) Volcano plots of differentially expressed genes (DEGs) between the COL and DP groups. (H) Gene Ontology (GO) enrichment analysis.(I) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.