FIGURE 4 Macrophages polarized in response to DP scaffold
promote BMSC migration and differentiation in vitro.
The viability of BMSCs after culture in different types of conditioned
medium (CM) for 1 and 3 days. (B&C) Representative images
and quantitative analysis of BMSC migration after culture in different
CM for 1 day. Scale bar, 50 μm. (D&E) Transwell-based BMSC
migration assays and quantification analysis. Scale bar, 1 mm.(F) ARS staining on the 21th day in
culture. (G) Quantitative results of ARS staining on the
21th day culture. (H) mRNA levels ofRunx2 and BMP2 in BMSCs after culture in different CM as
quantified by qRT-PCR.