2.5 Blood pressure and heart rate detection
The Dataquest ART system by Data Sciences International was utilized for real-time monitoring of blood pressure and heart rate for 24 hours on day 1, 7, 14, 21, and 28 after administering, with 11:00 AM data serving as a baseline each day (refer to Figure 1). The analysis of blood pressure was performed using Ponemah Software 5.0 from Data Sciences International, and the MAP (Mean Arterial Pressure) was calculated using the following formula: MAP = (SBP + 2*DBP) / 3.
2.6 Water consumption, natriuresis and diuresis detection
Water consumption and urinary output were measured using a metabolic cage. Natriuresis and diuresis were assessed on day 1, 4, and 28, 6 hours after dosing. Natriuresis was quantified using Na+ test kits and a fully automatic biochemical instrument from HITACHI.