Figure 2 : (a)Decrease in the absorbance of TMB at 450 nm and an increase in the
absorbance at 652/370 nm indicates the formation of a charge transfer
complex due to TMAO. Inset of the figure is the equilibrium shift from
the yellow structure to the charge transfer complex in blue.(b) Calibration plot.
Interference
study
- Trimethylamine interference
TMAO detection depends on the formation of the charge transfer complex
(Scheme 2, 1 ). Amines are well-known quenchers and interfere
with forming charge transfer complexes (Young, Martin, Feriozi, Brewer,
& Kayser, 1973). Thus, trimethylamine may interfere with forming the
charge transfer complex necessary for TMAO detection.