3.3. Effect of UVB irradiation on enzymatic and
non-enzymatic assays
LPO estimation- Thyroid MDA levels in the UVB-irradiated group
were considerably higher than those in the control group. When compared
to the UVB-irradiated group, the distribution of Naringin to the UVB+NG
treated group resulted in a considerable drop in MDA levels (Fig.4.A).
SOD estimation- In comparison to the control group, the
UVB-irradiated group’s SOD activity was significantly higher. When
Naringin was administered to the UVB+NG treated group, the activity of
SOD was markedly reduced in comparison to the UVB irradiated group
(Fig.4.B).
Catalase activity- The UVB-irradiated group had significantly
more catalase activity than the control group. The endogenous
accumulation of hazardous H2O2 brought on by the lack of catalase
activity is predicted to cause a multitude of genetic modifications,
including single-strand and double-strand breaks, which may eventually
result in the pathological illness. The catalase level was found to be
lower in the UVB+NG treated group than it was in the UVB irradiated
group after naringin administration (Fig.4.C).
H2O2 activity- When compared to the control group, the H2O2
activity seen in the UVB-irradiated group was significantly higher. When
naringin was administered to the UVB+NG treated group, the activity of
H2O2 was markedly reduced in comparison to the UVB irradiated group
(Fig.4.D).
NO activity- NO activity was substantially higher in the
UVB-irradiated group than it was in the control group. When Naringin was
administered to the UVB+NG treated group, it was observed that the NO
level was lower than in the UVB alone group (Fig.4.E).
GR activity- In comparison to the control group, the GR
activity was significantly higher in the UVB-irradiated group. When
Naringin was administered to the UVB+NG treated group, the activity of
GR was markedly reduced in comparison to the UVB irradiated group
(Fig.4.F).
GSH activity- When compared to the control group, the GSH
activity seen in the UVB-irradiated group was significantly lower. When
naringin was administered to the UVB+NG treated group, the activity of
GSH was markedly increased in comparison to the UVB irradiated group
(Fig.4.G).
Changes in the ROS level- Reactive oxygen species is commonly
employed as a marker to detect tissue injury in toxicological and
clinical chemistry. Variations in ROS level are a sign of antioxidant
enzymes alterations occurring in the afflicted tissue. In comparison to
the control group, the ROS activity was significantly higher in the
UVB-irradiated group. When Naringin was administered to the UVB+NG
treated group, the activity of ROS was markedly reduced in comparison to
the UVB irradiated group (Fig.4.H).