2.13. Histopathological Analysis
Through the heart, 0.02M phosphate-buffered saline (PBS) and 4% PFA were used to perform whole-body perfusion.25 After being removed, the thyroid gland was placed in the fixative for two days. For haematoxylin and eosin staining, fixed tissues were treated, dried, and cut into 5 µm slices from paraffin-embedded thyroid. 10X and 40X magnification microscope analyses of images.