2.5. Study test design
The animals were randomly divided into four groups with six mice in each group. A timeline of dosage and experimental test design was portrait in Fig. 1.
Group 1: The animals received standard food and water ad libitum for 15 days (control group).
Group 2: The animals was exposed to UVB irradiation at 280 nm for two hours every day for 15 days.
Groups 3: The animals exposed to UVB irradiation along with Naringin (at a concentration of 80 mg/kg body weight) for 15 days orally.14
Group 4: The animals only received NG (at a concentration of 80 mg/kg body weight) for 15 days orally.
Throughout the therapy, body weight and daily food and drink intake were noted. Animals were starved overnight and then killed by cervical decapitation after the study period. After the connective tissues around the thyroid gland were removed, the thyroid gland was removed through a transverse abdominal incision and weighed.
2.6. Sample collection
After being extracted, whole blood samples were put in evacuated tubes containing an EDTA anticoagulant solution. The plasma obtained after centrifuging the second tube at 2200 rpm for 15 minutes was stored at -20°C until the levels of the thyroid hormones were determined.