3.6. Immunohistochemistry of Thyroid gland showing expression of THRβ-1, Caspase-3 and NOS-2
There was a marked reduction in the expression of THRβ-1 receptor in the thyroid of UVB exposed mice as compared to control. The thyroid of naringin treated showed similar TRHβ-1 receptor expression like that in the thyroid of control rats (Fig. 7). Moreover, the level of caspase-3 protein expression was significantly increased in UVB exposed mice compared to the expression in control. The intensity of activated caspase-3 immunostaining (deep brown) is pre-dominant on thyroid follicular disruption (Fig. 8). The expression of NOS-2 was seen to be increased in UVB exposed group as compared to control. The group of naringin treated showed the intensity of NOS-2 antibody somewhat similar to control (Fig. 9).