Figure 1 Sugar preference test
Note: Compared with the CON+NS group: *P <0.05, **P <0.01; compared with the MS+NS group:#P <0.05,##P <0.01.
3.1.2 Effect of probiotics on the autonomous activity of mother-infant separated offspring depression-like rats
(1) Central activity time
The open field experiment was to detect spontaneous activity, exploration, and anxiety-like behavior in rats. The results showed that the central activity time in the MS+NS group decreased comparedwith the CON+NS group, and the difference was statistically significant (P <0.01); the central activity time in the MS+P group increased compared with the MS+NS group, and the difference was statistically significant (P <0.05); the central activity time in the SD rats in the CON+NS group did not change significantly compared with the MS+P group (P >0.05). Figure 2(A).
(2) Central activity distance
The open field experiment was to detect spontaneous activity, exploration, and anxiety-like behavior of rats, and the results showedthat the central activity distance of MS+NS group decreased compared with CON+NS group, and the difference was statistically significant (P <0.01); the central activity distance of MS+NS group increased compared with MS+P group, and the difference was statisticallysignificant (P <0.01); compared with MS+P group, the central activity distance of CON+NS group There was no significant change in the central activity distance of SD rats compared with the MS+P group (P >0.05). Figure 2(B).
(3) Total distance travelled
The open field experiment was to detect spontaneous activity, exploration, and anxiety-like behavior of rats, and the results showed that the total distance traveled in the MS+NS group decreased compared with the CON+NS group, and the difference was statistically significant (P <0.05); the total distance traveled in the MS+P group increased compared with the MS+NS group, and the difference was statistically significant (P <0.05); compared with the MS+P group,the total distance traveled in the CON+NS group of SD rats There was no significant change in the total distance traveled in the CON+NS group compared with the MS+P group (P >0.05). Figure 2(C).