Differentially abundant ASVs
Across all the plots, sample ASV relative abundances (%) and corresponding log-fold-change results from ANCOM-BC differential abundance testing were highlighted for the top 50 ASVs in each plot that displayed the largest absolute magnitudes of log-fold change between comparison groups (see Figures S2-S5; Tables S3-S6). These most fluctuating ASVs were predominantly from the phyla Acidobacteriota, Actinobacteriota and Proteobacteria (Tables S3-S6). Respective total numbers of taxa from each plot with at least one occasion of significant differential abundance at later measurement times (06:00, 12:00, 18:00) compared to the midnight (00:00) baseline/intercept were 853 ASVs at the cleared Kenneth Stirling CP, 598 ASVs at native Kenneth Stirling CP, 922 ASVs at cleared Mark Oliphant CP, and 685 ASVs at the native Mark Oliphant CP plot. Time-series changes in relative abundance (%) of these differentially abundant ASVs over the sampling times were also visualised for each site (Figure S6). Non-trivial cohorts of taxa appeared to be varying temporally over the 24-hour period, and these fluctuating taxa were largely plot-specific (see Venn diagrams, Figure S7). Within a plot, numbers of taxa typically in the range of 200-400 ASVs were either increasing or decreasing when comparing baseline midnight (00:00) samples to later measurement times (06:00, 12:00, 18:00). Very few of the ASVs displaying temporal differential abundance were shared across plots, with approximately 1-4% of all differentially abundant (or in the order of 10-50 ASVs) shared between sites.