Differentially abundant ASVs
Across all the plots, sample ASV relative abundances (%) and
corresponding log-fold-change results from ANCOM-BC differential
abundance testing were highlighted for the top 50 ASVs in each plot that
displayed the largest absolute magnitudes of log-fold change between
comparison groups (see Figures S2-S5; Tables S3-S6). These most
fluctuating ASVs were predominantly from the phyla Acidobacteriota,
Actinobacteriota and Proteobacteria (Tables S3-S6). Respective total
numbers of taxa from each plot with at least one occasion of significant
differential abundance at later measurement times (06:00, 12:00, 18:00)
compared to the midnight (00:00) baseline/intercept were 853 ASVs at the
cleared Kenneth Stirling CP, 598 ASVs at native Kenneth Stirling CP, 922
ASVs at cleared Mark Oliphant CP, and 685 ASVs at the native Mark
Oliphant CP plot. Time-series changes in relative abundance (%) of
these differentially abundant ASVs over the sampling times were also
visualised for each site (Figure S6). Non-trivial cohorts of taxa
appeared to be varying temporally over the 24-hour period, and these
fluctuating taxa were largely plot-specific (see Venn diagrams, Figure
S7). Within a plot, numbers of taxa typically in the range of 200-400
ASVs were either increasing or decreasing when comparing baseline
midnight (00:00) samples to later measurement times (06:00, 12:00,
18:00). Very few of the ASVs displaying temporal differential abundance
were shared across plots, with approximately 1-4% of all differentially
abundant (or in the order of 10-50 ASVs) shared between sites.