Recording and processing of microbubbles
In the current setting, these bubbles in images were magnified by a Zeiss stereo microscope lens assembly achieving 50 × optical magnification with a depth of field of 86 μm, the lateral spatial resolution is 2.00 mm, and the vertical resolution is 2.45 mm. In order to obtain accurate statistics, a total of 1600 images in each measurement were collected. The images were post-processed and analyzed using the software Davis (Lavision Germany). First, the original image was normalized and noise-filtered, and then particle detection with binarization was performed. The parameters for image processing and particle recognition can be set in the Analyzer settings panel, and the parameters are summarized in Table 1.