Recording and processing of microbubbles
In the current setting, these bubbles in images were magnified by a
Zeiss stereo microscope lens assembly achieving 50 × optical
magnification with a depth of field of 86 μm, the lateral spatial
resolution is 2.00 mm, and the vertical resolution is 2.45 mm. In order
to obtain accurate statistics, a total of 1600 images in each
measurement were collected. The images were post-processed and analyzed
using the software Davis (Lavision Germany). First, the original image
was normalized and noise-filtered, and then particle detection with
binarization was performed. The parameters for image processing and
particle recognition can be set in the Analyzer settings panel, and the
parameters are summarized in Table 1.