2.4 RT-qPCR analysis
Total RNA was extracted using a Foregene Plant RNA Isolation Kit
(Foregenes, Chengdu, China). The first-strand cDNA was synthesized via a
Thermo Scientific Kit (Thermo Scientific, Carlsbad, CA, USA). RT-qPCR
was conducted with a Roche LightCycler® 96 Real-Time
PCR System (Roche, Indianapolis, IN, USA). The malate
dehydrogenase (MdMDH ) gene in M. domestica was used to
normalize the expression of different genes. The primers used are listed
in Table S1.