2.9 Dual-luciferase reporter assay
MdNRT2.1 and MdNRT2. 4 promoters were cloned into pGreenII 0800-LUC vectors and generated the reporter constructMdNRT2.1 pro:LUC and MdNRT2.4 pro:LUC. The CDS of theMdHY5 was cloned into the pGreenII 62-SK vector and generated the effector 35Spro:MdHY5 . LUC/REN activity was evaluated using a Dual-Luciferase® reporter gene assay kit (Yeasen Biotechnology, Shanghai).